干细胞与转化医学实验室
一、主要研究内容
1、人多功能干细胞定向分化肝细胞、造血干细胞、血小板及其机理研究，干细胞在组织器官的功能修复与替代的作用，干细胞源的功能细胞在生物医药的应用。
2、基于人多能干细胞的类器官的构建和应用，构建了人胚胎干细胞来源的肝祖细胞类器官、成熟肝细胞类器官、造血类器官、胸腺类器官及肿瘤干细胞来源的肝癌血管化的类器官。
3、肿瘤干细胞的转化机理及其在临床上促发肿瘤发生发展、转移和复发中的作用研究，研制新一代包括清除肿瘤干细胞的癌症治疗方法究。
利用干细胞培养技术，建立了在体外克隆化培养肿瘤干细胞且维持其干性的技术；这一技术有助于从临床样品中建立人类主要恶性肿瘤的肿瘤干细胞库，这对肿瘤的发生发展、转移和复发、清除肿瘤干细胞（肿瘤的种子细胞）的基础和临床研究具有重要意义。
4、基于干细胞或基于干细胞产物的体内组织和体外组织损伤修复和再生。
二、实验室PI介绍
段玉友, 自2022年11月担任华南理工大学附属第二医院特聘研究员、硕/博士生导师、博士后合作导师，干细胞与转化医学实验室PI。曾在华南理工大学医学院（生命科学研究院）担任特聘研究员（2017-2022）、美国加州大学戴维斯分校担任副教授、助理教授、项目科学家、职员研究员、博士后研究员（2000-2017）、康奈尔大学任职博士后研究员（1999-2000）。1988年至2004年在美国康奈尔大学、加州大学戴维斯分校、扬州大学从事教学和科研工作，研究主要从事肿瘤病毒、传染性病原研究，包括致病机制、病原诊断及检测、疫苗研制及疾病预防。自2002年始至今，在加州大学戴维斯分校、华南理工大学、华南理工大学附属第二医院从事教学和科研，研究主要从事干细胞再生医学、转化医学、肿瘤干细胞生物学、肝脏疾病及再生、组织损伤修复和组织工程、生物信息在生物医药的应用等方向的研究。
回国任职以来主持国家重点研发项目课题1项、国家重点研发项目子课题2项，曾作为主持人、共同主持人承担美国NIH、加州再生研究所（CIRM）、国际合作项目、加州大学戴维斯分校基金等多项课题。在Gastroenterology、Journal of Nanobiotechnology、Stem Cell Research and Therapy、Liver International等期刊发表论文80余篇。曾获加州大学戴维斯分校医务系统优秀成果奖，教育部科技进步二等奖，江苏省科技进步四等奖，江苏省高等学校优秀青年骨干教师称号等。二十多个SCI杂志审稿人，中国实验动物小型猪专业委员会委员，广东省医学会细胞治疗分会委员会委员。
三、发表论文（selected）
1. Shoupei Liu, Jue Wang, Sen Chen, Zonglin Han, Haibin Wu, Honglin Chen*, Yuyou Duan*. C/EBPβ coupled with E2F2 promoted the proliferation of hESC-derived hepatocytes through directly binding to the promoter regions of cell cycle-related genes. Cells 2023; 12(3):497.
2. Yan Liang#, Sen Chen#, Jinghe Xie#, Guanrong Yan, Tingting Guo, Tianyang Li, Shoupei Liu, Weiping Zeng, Shuai Zhang, Keqiang Ma, Yimeng Ou, Bailin Wang, Weili Gu, Honglin Chen, Yuyou Duan*. Establishment of a prognostic model based on m6A regulatory factors and stemness of hepatocellular carcinoma using RNA-seq data and scRNA-seq data. Journal of Cancer Research and Clinical Oncology 2023; 149(14):12881-12896.
3. Zhengbo Wen, Minmin Zhang, Yuxin Chen, Peilin Liao, Fengyu Wang, Shoupei Liu, Haibing Wu, Jinghe Xie, Jue Wang, Ning Wang, Yingjie Fu, Lorenzo Moroni, Yuyou Duan*, Honglin Chen*. In situ precision cell electrospinning as an attractive stem cell delivery approach for cutaneous wound healing. Advanced Health Materials 2023, 2300970.
4. Lingfeng Xiong, Yongping Luo, Tianbai Yuan, Weipeng Lin, Bohui Lin, Chen Wu, Yuyou Duan*, Yimeng Ou. Prognostic 7-SLC-Gene Signature Identified via Weighted Gene Co-expression Network Analysis for Patients with Hepatocellular Carcinoma. Journal of Oncology 2023; 2023: Article ID 4364654.
5. Jiamin Lin#, Pingping Liu Wei#, Guorong Liu, Juan Zhang, Min Yan*, Yuyou Duan*, Na Yang*. A positive feedback loop between ZEB2 and ACSL4 regulates lipid metabolism to promote breast cancer metastasis. eLife 2023, Accepted
6. Jue Wang#, Ping Situ#, Sen Chen#, Haibin Wu, Shoupei Liu, Xueyan Zhang, Yiyu Wang, Jinghe Xie, Honglin Chen*, Yuyou Duan*. Hepatic polarized differentiation promoted the maturity and liver function of human embryonic stem cell-derived hepatocytes via activating Hippo and AMPK signaling pathways. Cells 2022; 11(24): 4117.
7. Haibin Wu#, Xianglian Tang#, Yiyu Wang, Ning Wang, Qicong Chen, Jinghe Xie, Shoupei Liu, Zhiyong Zhong, Yaqi Qiu, Ping Situ, Mark A . Zern, Jue Wang, Honglin Chen*, Yuyou Duan*. Dextran sulfate prevents excess aggregation of hPSCs in 3D culture by inhibiting ICAM1 expression coupled with down-regulating E-cadherin through activating the Wnt signaling pathway. Stem Cell Research & Therapy 2022;13:218.
8. Weiping Zeng, Zhengbo Wen, Honglin Chen*, Yuyou Duan*; Exosomes as carriers for drug delivery in cancer therapy. Pharmaceutical Research 2022, 40(4):873-887.
9. Ning Wang#, Chuxin Chen#，Yang Cheng#, Yinjie Fu, Zhiyong Zhong, Honglin Chen*, Jian Huang*, Yuyou Duan*. Hypoxia drives hematopoiesis with the enhancement of T lineage through eliciting arterial specification of hematopoietic endothelial progenitors from hESCs. Stem Cell Research & Therapy 2022; 13:282.
10. Xiajing Li, Yiyu Zhang, Ning Wang, Zhaohu Yuan, Xiaojie Chen, Qicong Chen, Hui Deng, Xinxin Tong, Honglin Chen, Yuyou Duan*, Yaming Wei*. CircRNA.0007127 triggered the apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells. Journal of Zhejiang University-SCIENCE B 2022; 23(9):732-746.
11. Jinghe Xie, Yaqi Qiu, Shuai Zhang, Keqing Ma, Yimeng Ou, Bailin Wang, Weili Gu, Honglin Chen and Yuyou Duan*. Identification of factors regulating the gene expression of alcohol dehydrogenases in hepatocellular carcinoma. Journal of Drug and Alcohol Research 2022;11:Article ID 236157.
12. Jiayi Zhang, Minglu Zhong, Zhaohu Yuan, Weijie Zhong, Yanfei Lan, Yuyou Duan*, Yaming We*. Construction of Tandem Diabody (IL-6/CD20)-Secreting Human Umbilical Cord Mesenchymal Stem Cells and Its Experimental Treatment on Diffuse Large B-Cell Lymphoma. Stem Cell Research & Therapy 2022; 13:473.
13. Lingfeng Xiong, Yongping Luo, Tianbai Yuan, Weipeng Lin, Bohui Lin, Chen Wu, Yuyou Duan*, Yimeng Ou. Prognostic 7-SLC-Gene Signature Identified via Weighted Gene Co-expression Network Analysis for Patients with Hepatocellular Carcinoma. Journal of Oncology 2023; 2023: Article ID 4364654.
14. Ning Wang#, Xiajing Li,# Zhiyong Zhong, Yaqi Qiu, Shoupei Liu, Haibin Wu, Xianglian Tang, Chuxin Chen, Yingjie Fu, Qicong Chen, Tingting Guo, Jinsong Li, Shuai Zhang, Mark A Zern, Keqing Ma, Bailin Wang Yimeng Ou, Weili Gu*, Jie Cao*, Honglin Chen*, Yuyou Duan*. 3D hESC exosomes enriched with miR-6766-3p ameliorates liver fibrosis by attenuating activated stellate cells through targeting the TGFβRII-SMADS pathway. Journal of Nanobiotechnology 2021;19:437.
15. Qiu Y#, N Wang#, T Guo,# S Liu, X Tang, Z Zhong, Q Chen, H Wu, X Li, J Wang, S Zhang, Y Ou, B Wang, K Ma, W Gu*, J Cao*, H Chen*, Y Duan*. Establishment of a 3D model of tumor-driven angiogenesis to study the effects of anti-angiogenic drugs on pericyte recruitment. Biomaterials Science 2021, 9:6064-6085.
16. Tang X#, H Wu#, J Xie, N Wang, Q Chen, Z Zhong, Y Qiu, J Wang, X Li, P Situ, L Lai, MA Zern, H Chen, Y Duan*. The combination of dextran sulfate and polyvinyl alcohol prevents excess aggregation and promotes proliferation of pluripotent stem cells in suspension culture. Cell Proliferation 2021;54:e13112.
17. Jinghe Xie, Tingting Guo, Zhiyong Zhong, Ning Wang, Yan Liang, Weiping Zeng, ShouPei Liu, Qicong Chen, Shuai Zhang, Keqing Ma, Bailin Wang, Yimeng Ou, Weili Gu*,Honglin Chen*, Yaqi Qiu*, and Yuyou Duan*. ITGB1 drives hepatocellular carcinoma progression by modulating cell cycle process through PXN/YWHAZ/AKT pathways. Frontiers Cell and Developmental Biology 2021; 9:Article 711149.
18. Chen J, B Tschudy-Seney, MA Zern*, P Liu*, Y Duan*. Enhancement of hepatocyte differentiation from human embryonic stem cells by Salvianolic acid B. Stem Cells Dev 2018; 27(4):252-261.
19. Chen J, Chen L, Zern MA, Thesie ND*, Diehl AM*, Liu P*, Duan Y*. The diversity and plasticity of adult hepatic progenitor cells and their niche. Liver International 2017; 37(9):1260-1271.
20. Chen J, Gao W, Zhou P, Ma X, Tschudy-Seney B, Liu C, Zern MA*, Liu P*, Duan Y*. Enhancement of hepatocyte differentiation from hESC by Chinese medicine Fuzheng Huayu. Sci Rep 2016; 6; 6:18841.
21. Zeng C, Y Zhang, S Park, J Eun J, N Nguyen, Y Jung, B Tschudy-Seney, ND Theise*, MA Zern*, Y Duan*. CD34+ Liver Cancer Stem Cells were Formed by Fusion of Hepatobiliary Stem/Progenitor Cells with Hematopoietic Precursor-Derived Myeloid Intermediates. Stem Cells Dev 2015;24(21):2467-78.
22. Park S, C Zeng, N Nguyen, J Eun, Y Zhang, MA Zern*, Y Duan*. Clonogenically culturing and expanding CD34+Liver cancer stem cells in vitro. Stem Cells Dev, 2015; 24(13):1506-14.
23. Park S, N Nguyen, J Eun, Y Zhang, Y Jung, B Tschudy-Seney, A Trotsyuk, A Lam, R Ramsamooj, ND Theise, MA Zern*, Y Duan*. Identification of cancer stem cell subpopulations of CD34(+) PLC/PRF/5 that result in three types of human liver carcinomas. Stem Cells Dev 2015; 24(8):1008-21.
24. Eun J, Y Jung, Y Zhang, Y Zhang, B Tschudy-Seney, R Ramsamooj, Y Wan, ND Theise, MA Zern, Y Duan*. Hepatoma SK Hep-1 cells exhibit characteristics of oncogenic mesenchymal stem cells with highly metastatic capacity. PLOS ONE 2014; 9(10):e110744.
25. Gao W, P Zhou, X Ma, B Tschudy-Seney, J Chen, Y Zhang, NL Magner, MA Zern, Y Duan*. Ethanol negatively regulates hepatic differentiation of hESC by inhibition of the MAPK/ERK signaling pathway. PLOS ONE 2014; 9(11):e112698.
26. Ma, X., Y Duan#*, B Tschudy-Seney, G Roll, I Saramipoor Behbahan, T Ahuja, V Tolstikov, C Wang, J McGee, S Khoobyari, J Nolta, H Willenbring, MA Zern*. Efficient differentiation of functional hepatocytes from human induced pluripotent stem cells. Stem Cells Translational Medicine 2013; 2:409-419.
27. Ghaedi M, Soleimani M, Shabani I, Duan Y*, Lotfi A*. Hepatic differentiation from human mesenchymal stem cells on a novel nanofiber scaffold. Cell Mol Biol Lett 2012; 17:89-106.
28. Duan Y, X Ma, W Zou, C Wang, I Behbahan, T Ahuja, V Tolstikov, MA Zern*. Differentiation and characterization of metabolically functioning hepatocytes from human embryonic stem cells. STEM CELLS 2010, 28:674-686.
29. Duan Y, A Canata, Y Meng, N Yamamoto, S He, S Gupta, SS Gambhir and MA Zern*. Differentiation and enrichment of hepatocyte-like cells from human embryonic stem cells in vitro and in vivo. STEM CELLS 2007, 25:3058-3068.
30. Duan Y, J Wu, J Zhu, S Liu, I Ozaki, D Strayer, and MA Zern*. Gene therapy for human alpha 1-Antitrypsin deficiency in an animal model using SV40-derived vectors. Gastroenterology 2004, 127:1222-1232.